Jaleh Ghashghaie

Position: Professor

Team: Plant Ecophysiology

Contact details:
Laboratoire Écologie, Systématique et Évolution – IDEEV
Bureau 2212 Bât. 680 – 12, route 128
91190 Gif Sur Yvette

Tel: +33 (0)1 69 15 63 59

Fax: +33 (0)1 69 15 72 38

Email: jaleh.ghashghaie (at) universite-paris-saclay.fr

Laboratoire Ecologie, Systématique et Evolution

Research interests

The main focus of my research work is to study the respiratory metabolism of plants using stable carbon isotopes. The aim is to determine the isotopic discrimination during respiration to understand the metabolic origin of the C released by the respiration of plants subjected to different environmental conditions (temperature, drought, N nutrition, etc.). Therefore, we measure the natural abundance in 13C/12C in the CO2 evolved during respiration of leaves and roots of different species (C3 and C4) as well as in the putative respiratory substrates (mainly soluble sugars and organic acids). These measurements are also carried out using (i) the 13C-labeled CO2 to label the photoassimilates and then to follow the labeled C in respired CO2 and in metabolites, and (ii) the positionnally labeled metabolites for example glucose or pyruvate labelled in C atom position C-1, C-2 or C-3, etc. We have already demonstrated that, contrary to what was assumed until recently, CO2-respired by leaves is enriched in 13C, while that released by roots is depleted in 13C, compared with the metabolites in corresponding organs. This is validated in both C3 and C4 herbs and we demonstrated the metabolic origin of this opposite isotopic discrimination between leaf and roots.

Currently, I study the impact of the inorganic nitrogen source (NO3-versus NH4 +) on the metabolism of sugars and organic acids and respiratory metabolism, and thus on the 13C/12C composition of respired CO2 and respiratory substrates. Since NO3-is mainly assimilated in the leaves, while NH4 + is preferentially assimilated in the roots, their assimilation would affect the anaplerotic activity of the enzyme PEPc (which enriches in 13C the C fixed), it is expected that the metabolites and the CO2 respired by these organs will be differently impacted under nitrogen nutrition with various NO3-: NH4 + ratios in supplied N (a PhD thesis in progress).

Laboratoire Ecologie, Systématique et Evolution


Stable isotopes: Use of stable isotopes in ecophysiology (basic concepts on stable isotopes and isotope effects, isotopic discrimination during photosynthesis in C3, C4 and CAM plants and during respiration, isotope labelling, use at ecosystem level) (Lecture : License 3 and Master 2).

Drought resistance: Mechanisms of resistance/adaptation of plants to drought (morphological, structural and physiological adaptations) (Lecture: Master 1).

Soil: Physicochemical and biological properties of soil, degradation of organic matter in soil, mineralizations and humification, different types of humus and different types of soil (Lecture: License 3).

Water Relations: Leaf water potential and its components (measurement with pressure chamber), calculation of osmotic adjustment (Practical course: License 3).

Soil water: Measurements of soil properties related to water retention (total porosity, apparent density, field capacity, permanent wilt point, water reserve useful for plants) (Practical course: License 3).

Leaf gas exchanges: Measurement methods and calculations of different parameters (photosynthesis, transpiration, stomatal conductance, internal CO2 concentration, water use efficiency), limitations of the methods (problems related to heterogeneity of stomatal closure, mesophyll resistance, CO2 concentration in chloroplasts, cuticle resistance to water vapour diffusion (Course: License 3).

Laboratoire Ecologie, Systématique et Evolution

Career path

2009 – Present Professor of the University, Science Faculty of Orsay, University of Paris-Sud, France.
2004 Habilitation (HDR)
1991 – 2009 Assistant Professor, Science Faculty of Orsay, University of Paris-Sud, France.
1989 – 1991 Temporary Teaching and Research position (ATER), Science Faculty of Orsay, University of Paris-Sud, France.
1987 – 1988 Research contract, Moët-Hennessy Recherche, Paris.
1986 – 1987 Associated Assistant, Science Faculty of Orsay, University of Paris-Sud, France.
1986 PhD in Life Science, Science Faculty of Orsay, University of Paris-Sud, France.
1982 Master 2 in Plant Ecology, Science Faculty of Orsay, University of Paris-Sud, France.
1981 Master 1 Plant Ecology, Science Faculty of Orsay, University of Paris-Sud, France.
1980 French language Diploma, Centre of Audiovisual Studies of Languages (CAREL), Royan, France.
1979 License of Plant Biololgy, Faculty of Sciences, University of Tabriz, Iran.

Laboratoire Ecologie, Systématique et Evolution



Mirande-Ney C, Tcherkez G, Balliau T, Zivy M, Gilard F, Cui J, Ghashghaie J, Lamade E. (2020). Leaf metabolic effects of potassium fertilisation before visible changes in yield in oil palm (Elaeis guineensis Jacq.). Environmental & Experimental Botany, DOI: 10.1016/j.envexpbot.2020.104062


Mirande-Ney C, Tcherkez G, Gilard F, Ghashghaie J, Lamade E. (2019). Potassium fertilization affects oil palm fruit metabolism but has little effect on mesocarp lipid accumulation. Journal of Agricultural and Food Chemistry, DOI: 10.1021/acs.jafc.9b04336

Fila G, Zeinalipour N, Badeck FW, Delshad M, Ghashghaie J. (2019). Application of water-saving treatments reveals different adaptation strategies in three Iranian melon genotypes. Scientia Horticulturae. DOI: 10.1016/j.scienta.2019.05.045

Sarabi B, Fresneau C, Streb P, Citerne S, Badeck FW, Bolandnazar S, Ghaderi N, Tangama M, David A, Ghashghaie J.  (2019). Stomatal and non-stomatal limitations are responsible in down regulation of photosynthesis in melon plants grown under saline conditions: Application of carbon isotope discrimination as a proxy. Plant Physiology and Biochemistry DOI:10.1016/j.plaphy.2019.05.010


Khosravi E, Mousavi A, Farhadpour M, Ghashghaie J, Ghanati F, Haghbeen K. (2018)-Pyrrolizidine alkaloids-free extract from the cell culture of Lithospermum officinale with high antioxidant capacity. Applied Biochemistry and Biotechnology. http://doi.org/10.1007/s12010-018-2830-3

Bagues M, Sarabi B, Ghashghaie J, Souli I, Nagaz K. (2018)-Multivariate analysis of two barley landraces under deficit irrigation with saline water in southern Tunisia. Plant Biotechnology, 35:193-206.

Mihoub I, Badeck F-W, Aïd F, Lamothe-Sibold M, Ghashghaie J. (2018)-Relationships between taproot characteristics, carbon isotope composition and grain yield in four safflower accessions. Arid Land Research and Management, doi:10.1080/15324982.2018.1463574


Bathellier C., Badeck F-W., Ghashghaie J. (2017)-Carbon Stable Isotope Fractionation in Plant Respiration. In “Plant respiration: metabolic fluxes and carbon balance” (eds. G. Tcherkez, J. Ghashghaie), Vol. 43, Chapter 3. In Book Series Advances in Photosynthesis and Respiration (Series eds. Govindjee, T.D. Sharkey), Springer, Dordrecht, The Netherlands,

Sarabi B, Bolandnazar S, Ghaderi N, Ghashghaie J. (2017)-Genotypic differences in physiological and biochemical responses to salinity stress among melon (Cucumis melo L.) landraces.Plant Physiology and Biochemistry,119:294-311

Tcherkez G., Ghashghaie J. (Eds.) (2017)-Plant respiration: metabolic fluxes and carbon balance (2017). Book Series “Advances in Photosynthesis and Respiration” Vol. 43.Springer, Dordrecht, The Netherlands,

Zeinalipour N, Haghbeen K, Tavassolian I, Ghashghaie J. (2017)-Production of 3-methylthiopropionic ethyl ester in native Iranian Cucumis melo L. Group Dudaim under regulated deficit irrigation.Journal of Functional Foods,30:56-62


Ghashghaie J, Badeck FW, Girardin C, Huignard C, Aydinlis Z, Fonteny C, Priault P, Fresneau C, Lamothe-Sibold M, Streb P, Terwilliger VJ (2016)-Changes and their possible causes in d13C of dark-respired CO2 and bulk tissue of different organs of maize plants during early ontogeny.Journal of Experimental Botany,67(9):2603-2615

Imane Mihoub, Jaleh Ghashghaie, Franz W. Badeck, Thierry Robert, Marlène Lamothe-Sibold, and Fatiha Aid (2016)-Intraspecific variability of carbon isotope discrimination and its correlation with grain yield in safflower: prospects for selection in a Mediterranean climate.Isotopes inironmental and Health Studies,DOI: 10.1080/10256016.2016.1142

Mihoub I, Robert T, Ghashghaie J, Vilatersana R, Lamy F, Benmrid R, Lamothe-Sibold M, Aïd F.  (2016)-Phylogenetic position of two endemic Carthamus species in Algeria and their potential as sources of genes for water use efficiency improvement of safflower.Journal of Systematics and Evolution.,doi: 10.1111/jse.12220


Barzegar T., Delshad M., Kashi A.K., Mauve C, Ghashghaie J. (2015)-Sugar accumulation pattern and contents in developing fruits of two Iranian melon cultivars.Iranian Journal of Plant Physiology,5(3):1353-1359

Ghashghaie J., Badeck FW., Girardin C., Sketriené D., Lamothe M., & Werner R.A.  (2015)-Changes in d13C of dark-respired CO2 and organic matter of different organs of peanut plants during early ontogeny.Isotopes in Environment and Health Studies,51:93-108

Lamade E., Tcherkez G., Darlan N., Lobato Rodrigues R., Fresneau C., Mauve C., Lamothe M., Sketriené D., & Ghashghaie J.  (2015)-Natural 13C distribution in oil palm (Elaeis guineensis Jacq.) and consequences for allocation pattern.Plant, Cell & Environment,DOI: 10.1111/pce.12606.


Ghashghaie J. & Badeck FW (2014)-Opposite carbon isotope fractionation during dark respiration in roots vs leaves – A review.New Phytologist – Tansley review ,doi: 10.1111/nph.12563


Barzegar T., Badeck F.-W., Delshad M., Kashi A.-K. , Berveiller D. & Ghashghaie J. (2013)-13C-labelling of leaf photoassimilates to study the source-sink relationship in two Iranian melon cultivars.Scientia Horticulturae,151:157-164

Ghashghaie J. & Tcherkez G. (2013)-Isotope ratio mass spectrometry technique to follow plant metabolism: principles and applications of 12C/13C isotopes.Advances in Botanical Research,67:378-405


Ghashghaie J. (2012)-Réponses physiologiques des plantes aux facteurs environnementaux : (i) Activité photosynthétique (ii) Métabolisme respiratoire.Editions Universitaires Européennes,124 p. ISBN : 978-3-8417-9749-0

Rizza F., Ghashghaie J., Meyer S., Matteu L., Mastrangelo AM. &  Badeck FW. (2012)-Constitutive differences in water use efficiency and adaptive strategies in two durum wheat cultivars used as parents in a mapping population.Field Crop Research,125:49-60

Werner C., Schnyder H., Cuntz M., Keitel C., Zeeman M. Dawson T., Badeck FW., Brugnoli B., Ghashghaie J., Grams T., Kayler Z., Lakatos M., Dawson T., Máguas C., Ogée J., Rascher K., Siegwolf R., Unger S., Welker J., Wingate L. & Gessler A. (2012)-Progress and challenges using stable isotopes to trace plant carbon and water relations across scales.Biogeosciences.,9:3083-3111


Brüggemann N., Gessler A., Kayler Z., Keel S., Badeck FW., Barthel M., Boeckx P., Buchmann N., Brugnoli E., Esperschütz J., Gavrichkova O., Ghashghaie J., Gomez-Casanovas N., Keitel C., Knohl A., Kuptz D., Palacio S., Salmon Y., Uchida Y. & Bahn M. (2011)-Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuum: a review.Biogeosciences,8:3457-3489


Tcherkez G., Schäufele R., Nogués S., Piel C., Boom A., Lanigan G., Barbaroux C., Mata C., Elhani S., Hemming D., Maguas C., Yakir D., Badeck FW., Griffiths H., Schnyder H. & Ghashghaie J. (2010)-On the 13C/12C isotopic signal of day and night respiration at the mesocosm level.Plant, Cell & Environment ,33:900-913


Badeck F.W., Fontaine J.L., Dumas F. & Ghashghaie J.  (2009)-Consistent patterns in leaf lamina and leaf vein C isotope composition across 10 herbs and tree species.Rapid Communications in Mass Spectrometry,23:2455-2460

Bathellier C., Tcherkez G., Bligny R., Gout E., Cornic G. & Ghashghaie J. (2009)-Metabolic origin of the delta13C of respired CO2 in roots of Phaseolus vulgaris. New Phytologist,181(2):387-399

Bathellier C., Tcherkez G., Mauve C., Bligny R., Gout E. & Ghashghaie J. (2009)-On the resilience of nitrogen assimilation by intact roots under starvation, as revealed by isotopic and metabolomic techniques.Rapid Communications in Mass Spectrometry,23:2847-2856

Devaux M., Ghashghaie J., Bert D., Lambrot C., Gessler A., Bathellier C., Ogée J. & Lousteau D. (2009)-Carbon stable isotope ratio of phloem sugars in mature pine trees throughout the growing season: comparison of two extraction methods. Rapid Communications in Mass Spectrometry,23:2511-2519

Gessler A., Tcherkez G., Karyanto O., Keitel C., Ferrio JP., Ghashghaie J., Kreuzwieser J. & Farquhar GD.  (2009)-On the metabolic origin of the carbon isotope composition of CO2 evolved from darkened light-acclimated leaves in Ricinus communis. New Phytologist,181(2):374-386

Girardin C., Rasse D., Biron P., Ghashghaie J. & Chenu C. (2009)-A method for 13C-labeling of metabolic carbohydrates within French bean leaves (Phaseolus vulgaris L.) for decomposition studies in soilsRapid Communications in Mass Spectrometry,23 (12):1792-1800

Lamade E., Setyio I. E., Girard S. & Ghashghaie J. (2009)-Changes in 13C/12C of oil palm leaves to understand the use of carbon during their passage from heterotrophy to autotrophy.Rapid Communications in Mass Spectrometry,23:2586-2596

Maseyk K., Seibt U., Ghashghaie J., Bathellier C., Almeida P., Lobo de Vale R., Yakir D., Pereira J.S., Mencuccini M. & Wingate L. (2009)-Biotic and abiotic factors affecting the d13C of soil respired CO2 in a Mediterranean oak woodland.Isotopes in Environmental & Hearth Studies,45 (4):343-359

Maunoury-Danger F., Bathellier C., Laurette J., Fresneau C., Ghashghaie J., Damesin C. & Tcherkez G. (2009)-Is there any 12C/13C fractionation during starch remobilisation and sucrose export in potato tubers?Rapid Communications in Mass Spectrometry,23:2527-2533

Mauve C., Bleton J., Bathellier C., Lelarge-Trouverie C., Guérard F., Ghashghaie J., Tchapla A. & Tcherkez G.  (2009)-Kinetic 12C/13C isotope fractionation by invertase: evidence for an in vitro isotope effect and comparison of 2 techniques for the isotopic analysis of carbohydrates. Rapid Communications in Mass Spectrometry ,23:2499-2506

Mortazavi B., Conte M., Chanton J. Smith M.C., Weber J.C., Crumsey J. & Ghashghaie J. (2009)-Does the 13C of foliage respired CO2 and biochemical pools reflect the 13C of recently assimilated carbon? Plant, Cell & Environment,32:1310-1323

Richter A., Waneck W., Werner R., Ghashghaie J., Jäggi M., Gessler A., Brugnoli E., Hettmann E., Göttlicher S.G., Salmon Y., Bathellier C., Kodama N., Nogués S., Soe A., Volder F., Sörgel K., Andreas Blöchl A., Siegwolf R., Buchmann N. & Gleixner G. (2009)-Preparation of starch and soluble sugars from plant material for analysis of carbon isotope composition: a comparison of methods.Rapid Communications in Mass Spectrometry,23:2476-2488


Bathellier C., Badeck F.W., Couzi P., Harscoët S., Mauve C. & Ghashghaie J.  (2008)-Divergence in ?13C of dark respired CO2 and bulk organic matter occurs during the transition between heterotrophy and autotrophy in Phaseolus vulgaris L. plants. New Phytologist,177(2):406-418

Gessler A., Tcherkez G., Peuke A.D., Ghashghaie J. & Farquhar G.D. (2008)-Experimental evidence for diel variations of the carbon isotope composition in leaf, stem and phloem sap organic matter in Ricinus communis. Plant, Cell and Environment,31:941-953


Fresneau C., Ghashghaie J. & Cornic G. (2007)-Drought effect on nitrate reductase and sucrose-phosphate synthase in wheat (Triticum durum L.): role of leaf internal CO2.Journal of Experimental Botany,58(11):2983-2992

Rizza, F. Matteu L., Ghashghaie J., Mastrangelo A., Stanca A. & Badeck FW.  (2007)-Water use and water use efficiency in two durum wheat cultivars contrasting for yield capacity.Photosynthesis Research,91 (2-3):319-320

Tcherkez G., Ghashghaie J. & Griffiths H. (2007)-Methods for improving the visualization and deconvolution of isotopic signals.Plant, Cell and Environment,30:887-891

Werner C., Unger S., Pereira J.S., Ghashghaie J. & Máguas C. (2007)-Temporal dynamics in ?13C of ecosystem respiration in response to environmental changes. In ‘Isotopes as Tracers of Ecological Change’, (Eds. T. Dawson & R. Siegwolf),ISBN: 978-012-373627-7. Elsevier Academic Press.,193-210


Fila G., Badeck F., Meyer S., Cerovic Z. & Ghashghaie J. (2006)-Relationships between leaf conductance to CO2 diffusion and photosynthesis in micropropagated grapevine plants, before and after ex vitro acclimatization. Journal of Experimental Botany,57(11):2687-2695

Griffiths H., Kromdijk W, Ghashghaie J., Tcherkez G. (2006)-Multidimensional isotopes: Integrating outputs from biological systems.Comparative Biochemistry and Physiology: A – Molecular & Integrative Physiology.,143(4):137

Nogués S., Damesin C., Tcherkez G., Maunoury F., Cornic G. & Ghashghaie J. (2006)13C/12C isotope labelling to study leaf carbon respiration and allocation in twigs of field grown beech trees.Rapid Communications in Mass Spectrometry,20:219-226

Nogués S., Tcherkez G., Streb P., Pardo A., Baptist F., Bligny R., Ghashghaie J. & Cornic G. (2006)-Respiratory carbon metabolism in the high mountain plant species Ranunculus glacialis. Journal of Experimental Botany ,57(14):3837-3845

Priault P., Tcherkez G., Cornic G., De Paepe R., Naïk R., Ghashghaie J. & Streb P.  (2006)-The lack of a functional mitochondrial complex I in a CMSII mutant of Nicotiana sylvestris increases photorespiration through an increased internal resistance to CO2 diffusion.Journal of Experimental Botany,57(12):3195-3207


Badeck F., Tcherkez G., Nogués S., Piel C. & Ghashghaie J. (2005)-Post-photosynthetic fractionation of stable carbon isotopes between plant organs – a widespread phenomenon. Rapid Communications in Mass Spectrometry,19:1381-1391

Tcherkez G., Cornic G., Bligny R., Gout E. & Ghashghaie J. (2005)-In vivo respiratory metabolism of illuminated leaves. Plant Physiology,138:1596-1606


Nogués S., Tcherkez G., Cornic G., Ghashghaie J. (2004)-Respiratory carbon metabolism following illumination in intact French bean leaves using 13C/12C isotope labelling.Plant Physiology,136:3245-3254

Tcherkez G., Farquhar G., Badeck F. , Ghashghaie J. (2004)-Theoretical considerations about carbon isotope distribution in glucose of C3 plants. Functional Plant Biology,31:857-877


Ghashghaie J., Badeck F.W., Lanigan G., Nogués S., Tcherkez G., Deléens E., Cornic G. & Griffiths H. (2003)-Carbon isotope fractionation during dark respiration and photorespiration in C3 plants. Phytochemistry Reviews,2:145-161

Tcherkez G., Nogués S., Bleton J., Cornic G., Badeck F.W. & Ghashghaie J. (2003)-Metabolic origin of carbon isotope composition of leaf dark-respired CO2 in Phaseolus vulgaris L.Plant Physiology,131:237-244


Duranceau M., Ghashghaie J. & Brugnoli E., (2001)-Carbon isotope discrimination during photosynthesis and dark respiration in intact leaves of Nicotiana sylvestris : Comparisons between wild type and mitochondrial mutant plantsAust. J. Plant Physiol.,28(1):65-71

Ghashghaie J., Duranceau M., Badeck M., Cornic G., Adeline M.T. & Deléens E.,  (2001)-?13C of CO2 respired in the dark in relation to ?13C of leaf metabolites : Comparison between Nicotiana sylvestris and Heliantus annuus under drought.Plant, Cell & Enviromnent,24:505-515

Ghashghaie J., Tcherkez G., Cornic G. & Deleens E., (2001)-Utilisation de la spectrométrie de masse isotopique en physiologie végétale.In : « Génomique fonctionnelle chez les végétaux : du gène à la fonction », Barbier-Brygoo H., Joyard J., Morot-Gaudry J.F. & Gaymard F. Eds, Presse INRA-CNRS.,97-105


Cornic G., Badeck F., Ghashghaie J. & Manuel N. (1999)-Effect of temperature on net CO2 uptake, stomatal conductance of CO2 and quantum yield of photosystem II photochemistry of deshydrated Pea leaves. In: « Crop development for the cool and wet regions of Europe ». M. Sanchez-Diaz et al.Eds., European Commission Press, Luxembourg.,287-297

Duranceau M., Ghashghaie J., Badeck F., Deleens E. & Cornic G. (1999)-Delta 13C of CO2 respired in the dark in relation to delta 13C of leaf carbohydrates in Phaseolus vulgaris L. under progressive drought. Plant Cell & Environment,22:515-523


Fila G., Ghashghaie J., Hoarau J. & Cornic G. (1998)-Photosynthesis, leaf condutance and water relations of in vitro cultured grapevine rootstock in relation to acclimatisation. Physiol. Plant.,102:411-418


Ghashghaie J. & Cornic. G. (1995)-In vivo estimation of CO2/O2 specificity factor of Rubisco at varying temperature. In “Photosynthesis: From Light to Biosphere.”, Vol. II (P. Mathis ed.)Kluwer Academic Publishers,927-930


Ghashghaie J. & Cornic G. (1994)-Effect of temperature on partitioning of photosynthetic electron flow between CO2 assimilation and O2 reduction and on the CO2O2 specificity of RubisCo estimated from gas exchange and chlorophyll measurements on intact leaves of 2 C3 plants. J. Plant. Physiol.,143:643-650


Cornic G., Ghashghaie J., Genty B. & Briantais J.M. (1992)-Leaf photosynthesis is resistant to a mild drought stress.Photosynthetica,27 (3):295-309

Ghashghaie J., Brenckmann F. & Saugier B.  (1992)-Water relations and growth of rose plants cultured in vitro under various relative humidities. Plant Cell Tissue & Organ Culture,30:51-57


Cornic G. & Ghashghaie J. (1991)-Effects of temperature on net CO2 assimilation and PSII quantum yield of electron transfert of French bean leaves (P. Vulgaris L.) during a drought stress.Planta,185:255-260

Ghashghaie J., Brenckmann F. & Saugier B. (1991)-Effects of agar concentration en water status and growth of in vitro Rose plants.Physiologia Plantarum,82:73-78

Mordacq L., Ghashghaie J. & Saugier B. (1991)-A simple field method for measuring gas exchange of small trees.Functional Ecology,5:572-576


Ghashghaie J. & Saugier B. (1989)-Effects of nitrogen deficiency on leaf photosynthetic response of tall fescue to water deficit.Plant Cell & Environment,12:261-271


Ghashghaie J. & Saugier B. (1988)-Photosynthetic response to water stress of tall fescue plants grown at two nitrogen levels. In: “Time Scale and Water Stress”. Proceedings of 5th International Conference on Mediterranean Ecosystems. Di Castri F., Floret Ch., Rambal S., Roy J. (Eds.)I.U.B.S., Paris.,81-85